Clinical chemistry 6 Electrophoresis


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Unformatted text preview: n mixtures and characterization of macromolecules (nucleic acids or proteins), based on the basis of size, electric charge, and other physical properties. Many important biological molecules such as amino acids, peptides, proteins, nucleotides, and nucleic acids, posses ionisable groups and, therefore, at any given pH, exist in solution as electrically charged species either as cations (+) or anions (­). Depending on the nature of the net charge, the charged particles will migrate either to the cathode or to the anode. How does this technique work? How does this technique work? Gel electrophoresis is a technique used for the separation of nucleic acids and proteins. Separation of large (macro) molecules depends upon two forces: charge and mass. When a biological sample, such as proteins or DNA, is mixed in a buffer solution and applied to a gel, these two forces act together. The electrical current from one electrode repels the molecules while the other electrode simultaneously attracts the molecules. The frictional force of the gel material acts as a "molecular sieve," separating the molecules by size. During electrophoresis, macromolecules are...
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