Clinical chemistry 6 Electrophoresis

Mechanismofelectrophoresis mechanismofelectrophoresis

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Unformatted text preview: ophoresis rely almost totally on the different charges on molecules to effect separation, while other methods exploit differences in molecular size and therefore encourage frictional effects to bring about separation. Mechanism of Electrophoresis Ez V= The velocity of migration (V) will depend on the 3 f factors: –E, or electric field strength; –z, or net charge on the molecules; –f, or frictional coefficient; V = Ez/f The velocity of migration of the molecules is directly proportional to the electric field strength (E) and the net charge of the molecules (z), and also inversely proportional to the frictional coefficient (f). Mechanism of Electrophoresis Mechanism of Electrophoresis Electric Field Strength, E – The greater the E, the faster that molecule will migrate; – Determined by the potential difference between the 2 oppositely charged electrodes. Net Charge on the Molecules, z – The greater the z on the molecules, the greater mobility it will have; – Dependent upon the total number of ionisable groups within the molecule (in protein, depends on the amine and carboxyl groups). Frictional Coefficient, f – The greate...
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