Clinical chemistry 6 Electrophoresis

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Unformatted text preview: lyacrylamide Gel By controlling the percentage (from 3% to 30%) of polyacrylamide, precise pore sizes can be obtained, usually from 5 to 2,000 Kdal. Tunnels/ Channels in the gel Polyacrylamide Gel, SDS­PAGE Polyacrylamide Gel, SDS­PAGE SDS coated proteins move toward anode; Smaller proteins migrate faster, since they can move through every tunnels; Larger proteins migrate slower, since they can only move through bigger tunnels; Different samples are loaded in wells or depressions at the top of the polyacrylamide gel. The proteins move into the gel when an electric field is applied. The gel minimizes convection currents caused by small temperature gradients, and it minimizes protein movements other than those induced by the electric field. Proteins can be visualized after electrophoresis by treating the gel with a stain such as Coomassie blue, which binds to the proteins but not to the gel itself. Each band on the gel represents a different protein (or a protein subunit); smaller proteins are found near the bottom of the gel. Loading Sample, SDS­PAGE Loading Sample, SDS­PAGE Equipments, SDS­PAGE Equipments,...
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