Clinical chemistry 6 Electrophoresis

Sds polyacrylamidegel polyacrylamidegel

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Unformatted text preview: continuous decrease in pore size from the top to the bottom of the gel, resulting in thin bands. Because of this banding effect, detailed genetic and molecular analysis can be performed on gradient polyacrylamide gels. – Polyacrylamide gels offer greater flexibility and more sharply defined banding than agarose gels. SDS­PAGE, Sodium dodecyl SDS­PAGE, Sodium dodecyl sulfate­polyacrylamide gel electrophoresis Gel Electrophoresis can be accomplished under Denaturing and Non­denaturing conditions; SDS­PAGE utilizes denaturing conditions where the proteins are denatured by boiling and subjected to Sodium dodecyl sulfate (SDS); SDS is an anionic detergent that binds to many sites on the protein obscuring the original charge, making the SDS­Protein complexes carry similar negative charge; Thus all SDS­Protein complexes migrate toward the positive and depend solely upon its size (or molecular weight, MW). Denaturation of proteins, SDS­ Denaturation of proteins, SDS­ PAGE Anionic surfactant wraps around polypeptide backbone; Negative charge with equal charge or charge densities per unit length; Separation NOT by charge but by MW! SDS Polyacrylamide Gel Po...
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