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Unformatted text preview: he movement of this charged molecule. This frictional force is a measure of the hydrodynamic size of the molecule, the shape of the molecule, the pore size of the medium in which electrophoresis's taking place and viscosity of the buffer. The velocity (v) of a charged molecule in an electric field is given by the equation: v = Eq / f where f is the frictional coefficient. In electrophoresis, the force moving the macromolecule (nucleic acids or proteins) is the electrical potential, E. The electrophoretic mobility (m) of an ion is the ratio of the velocity of the particle, v, to the electrical potential. m = v / E Electrophoretic mobility is also equal to the net charge of the molecule, Z, divided by the frictional coefficient, f. m = Z / f When a potential difference is applied, molecules with different overall charges will begin to separate due to their different electrophoretic mobilities. Even molecules with similar charges will begin to separate if they have different molecular sizes, since they will experience different frictional forces. Some forms of electr...
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This document was uploaded on 03/22/2014.
- Spring '14