Sheep Blood Lab 2 - Permeability of the red blood cell the...

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Permeability of the red blood cell– the rate of penetration of non-electrolytes using hemolysis By Lauren McKenzie 7275922 BIO1140 Section B1 Demonstrators: Rebecca Rochman and Hong Meng Yew February 3 rd , 2014 Department of Biology University of Ottawa
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Table 1. The mean observed hemolysis times for various non-electrolyte substances in sheep’s blood. The 5 solutions appear in no particular order. The concentration of each is 0.3M each. Sample sizes ( N ) are in parentheses and time is recorded in seconds (s). Time (s) Solution Mean (N) Standard Error Bi-distilled Water 2 a (3) 0 Glycerol 1209.67 (3) 167.44 Ethylene Glycol 15.25 (3) 0.80 Sucrose 1200 b (3) 0 Urea 5.24 (3) 0.88 a This mean does not represent the true value of the hemolysis time. It is a limitation of this experiment, as time less than two seconds cannot be measured. b This mean does not represent the true value of hemolysis time. It is a limitation to this
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Unformatted text preview: experiment as timing was stopped after 1200 seconds. Interpretation Questions 1. The factors that affect the diffusion of the solutes tested in the permeability experiment are the polarity, charge and the size of the solute molecules. 2. The more polar the solute, the harder it is for it to cross the membrane because the hydrophilic molecules have trouble passing through the hydrophobic, (or non polar part) of the lipid bilayer. This can be seen through the long hemolysis time of glycerol. Charged molecules cannot cross without the aid of a transport protein, as they cannot pass through the hydrophobic tail of the membrane. Additionally, the larger the solute, the more difficult it is to cross the membrane. In the case of the hemolysis in sucrose, it took a very long time (<1200 seconds), because carbohydrates are very big molecules....
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