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Unformatted text preview: is finished we have to fill in from this
point to the second OF, to this point of the first OF.
Like gap repair. The enzyme that gets rid of the primer
is a DNA/RNA hybrid. if you have DNA synthesis with
a RNA primer, we have to get rid of the primer.
RNASE-H degrades RNA. We remove the gap and
refill itand we get nicks- a single strand discontinuity,
all the bases have lined up but the phosphodiester
has not been made yet. The ligase creates that
phosphodiester bond. BIO 1140 Unit 2-1:Replication Enzyme Activities 3 11 Fig. 12.13, p. 272 BIO 1140 Unit 2-1:Replication Major Enzymes of DNA Replication ?
Many of these enzymes are
actually multiprotein complexes
12 Table 12.1, p. 273 at the end of the chromosomes, continuous
synthesis can take you to the end, but coming back
cannot take you back to the end. You always lose a
little bit that is opposite the primer. What happens
when you want DNA synthesis to go more quickly
and you wont faster DNA synthesis? BIO 1140 Unit 2-1:Replication Telomeres
Q? Ends of eukaryotic chromosomes. What happens to
them? Short sequences repeated hundreds to thousands of times (Humans have (TTAGGG)n ) Repeats protect against chromosome shortening during replication we cover the end with a simple sequence. Thia protects
against chromosome shortening. So we make some DNA
that doesn't count for anything, its just there to be thrown
away when the DNA shortens. Every generation, r
doubling, you replace it. The enzyme that adds this to the
end is called telome...
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- Winter '07