Dna enzymes go at a certain rate and we cannot make

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Unformatted text preview: ore proteins that recognize the origin are needed. 16 Fig. 12.16, p. 274 BIO 1140 Unit 2-1:Replication DNA REPLICATION IN BACTERIA: Overview The bacterial genome is a circular replicon. Replication initiates at a unique site, called the origin of Replication ( oriC) and proceeds in a bidirectional manner until the terminus is reached ( ter ). (these can be cloned and sequenced!!) oriC Did you know that E. coli cells can divide faster than they can replicate their DNA? ecoli has a circular with a single origin and termination. If the replication fork got bigger, it may be that have the chromosome was replicated. They are called theta structures. ter 17 Fig. 12-22 p. 280 BIO 1140 Unit 2-1:Replication How to synthesize a chromosome in a shorter time: Bacterial style Consider: Bacterial growth is assessed by the doubling time. E. coli can grow at rates ranging from 18 minutes to 180 minutes. The bacterial chromosome is a single replicon and the frequency of replication cycles is controlled by the number of initiation events at the single origin. Time to replicate a chromosome is fixed at about ~40 minutes. 18 UU U UU U From Genes VII: http://flylib.com/books/en/2.643.1.154/1/ ori UU ori U The solution is to reinitiate before termination! ori To solve this problem, ecoli initiates before it finishes. It inherits a molecule that is about 50% replicated and all it has to do is finish it off. BIO 1140 Unit 2-1:Replication Damage during synthesis...
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