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Unformatted text preview: ore proteins that recognize the
origin are needed. 16 Fig. 12.16, p. 274 BIO 1140 Unit 2-1:Replication DNA REPLICATION IN BACTERIA: Overview
The bacterial genome
is a circular replicon.
at a unique site,
called the origin of
Replication ( oriC)
and proceeds in a
until the terminus is
reached ( ter ).
(these can be cloned
and sequenced!!) oriC Did you know
that E. coli
cells can divide
DNA? ecoli has a circular with a single origin and
termination. If the replication fork got bigger, it may
be that have the chromosome was replicated. They
are called theta structures. ter 17 Fig. 12-22 p. 280 BIO 1140 Unit 2-1:Replication How to synthesize a chromosome in a shorter time:
Bacterial growth is assessed by the doubling time. E. coli can grow at rates ranging from 18 minutes to 180 minutes.
The bacterial chromosome is a single replicon and the frequency of replication
cycles is controlled by the number of initiation events at the single origin.
Time to replicate a chromosome is fixed at about ~40 minutes. 18 UU
U From Genes VII: http://flylib.com/books/en/2.643.1.154/1/ ori UU ori U The solution is to reinitiate before termination!
ori To solve this problem, ecoli initiates before it finishes.
It inherits a molecule that is about 50% replicated and
all it has to do is finish it off. BIO 1140 Unit 2-1:Replication Damage during synthesis...
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This note was uploaded on 03/26/2014 for the course BIO 1140 taught by Professor Fenwick during the Winter '07 term at University of Ottawa.
- Winter '07