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Unformatted text preview: mosomes: 1) use circular DNA b/c it has no end,
but we have linear ones and theres a solution. we have a RNA that makes the primer, RNA poymerase,
which can synthesis RNA denovo- doesnt need a primer
(spot can be defined) DNA polymerases: assemble nucleotides into a chain, remove
primers, and fill resulting gaps Specilaization DNA ligase: closes remaining single-chain nicks joins the gaps being filled (little pieces together) These enzymes work as multisubunit complexes
6 helicase-on the right a helix, on the left side, no helix and DNA unwinds from left to right BIO 1140 Unit 2-1:Replication Assembling Antiparallel Strands Leading strand=continuous
(notice the orientation) 7 Fig. 12-14, p. 271 BIO 1140 Unit 2-1:Replication the helix has opened up to allow two strand to have
DNA synthesis. We end up with two helices that are
antiparallel. Helicase functions at the replication fork.
Continuous synthesis- moves the helix towards the
right b/c the helix was opened up (leading strand).
Discontinuous-prime repeatedly to make the other
strand. The sizes of these regions are in the order of
500 to 1000 bps. The end result is ligation to join
fragments together. When making DNA, you make a
compliment to that strand. DNA synthesis actually
starts at the middle and goes both ways (bidirectional). DNA is synthesized 5' to 3', but we read
3' to 5'. Two Antiparallel Strands Helicase does not unwind the whole helix. As th...
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This note was uploaded on 03/26/2014 for the course BIO 1140 taught by Professor Fenwick during the Winter '07 term at University of Ottawa.
- Winter '07