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Unformatted text preview: e DNA helix unwinds, one template strand runs in a direction allowing new DNA strand to
be made continuously in the direction of
unwinding Other template strand is copied in short lengths that run in the direction opposite to unwinding
Discontinuous replication produces short lengths,
that are then ligated into a continuous strand
8 BIO 1140 Unit 2-1:Replication The helicase that unwinds uses ATP and has to be at
the replication fork. The RNA primer is made by
primase (any RNA polymerase that makes a primer).
These short (~1000 nts or
less) segments of RNA
Then the primase gets displace and the DNA
plus DNA are called
polymerase will come and copy. The little blue things
“Okazaki fragments” in
are single stranded binding proteins (SSBs). The
honour of their
discoverer Reiji Okazaki
proteins are there so that when it unwinds and
becomes single single stranded, it stays single
stranded. If not the polymerize could not read the
bases and synthesize the proper watson-crick htopoisomerase can relieve the stress of so
bonding pairs and also to make sure it doesnt fold up
much force at the end- where it is attached to histones.
into a secondary stucture- stem loop
Enzyme Activities 1 9 Fig. 12.13, p. 272 nicking closing enzyme, cuts the DNA, relieving stress and can ligase back together again (ex.gyrase). BIO 1140 Unit 2-1:Replication Enzyme Activities 2 10 Fig. 12.13, p. 272 We have to get rid of the okazaki fragments and once
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This note was uploaded on 03/26/2014 for the course BIO 1140 taught by Professor Fenwick during the Winter '07 term at University of Ottawa.
- Winter '07