Bio 1001 Lecture 9 Summary

Bio 1001 Lecture 9 Summary - Bio 1001 Tom Haffie LECTURE 9...

Info icon This preview shows pages 1–2. Sign up to view the full content.

Bio 1001 Tom Haffie LECTURE 9 DNA Damage vs. Mutation Damage affects only one strand, mutations affects BOTH strands Repair mechanisms DNA Polymerase roofreading --> misaligned base , can't add next nucleotide, uses 3'-->5' exonucluease activity Mismatch repair: 1) repair enzyme scan (mismatch distorts shape); 2) Exonuclueases remove DNA chunk 3) DNA polymerase extends strand 5' --> 3'; 4) Ligase seals strands Mobile (Transposable) Elements - -> TE's --> mutations by transposing into genes 1. Insertion Sequence (IS) --> has inverted repeats on each side o "Cut and Paste" --> Transposase cuts backbones of target sequence o "Copy and Paste" --> DNA Poly copies & Transposase cuts target sequence 2. Transposon --> IS sequence on both ends --> move everything in between --> antibiotic resistance in bacteria
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: 3. Retrotransposons --> RNA polymerase (RNA intermediate, benefit?) --> Reverse Transcriptase--> Transposase Retroviruses Direct, not inverted repeat sequences + genes code for: o Gag --> proteins for RNA core; Pol --> reverse transcriptase and Intergrase ; env --> viral coat proteins Endogenous --> stuck in genome (chicken pox?)--> creates junk in genome o Called provirus after inserted into genome (HIV is a retro virus, Influenza isn't) Determining TE age 1. Amount of mobile element copies in genome! 2. Finding transposon in other species 3. Number of mutations it has between (other species and/or within same species/genome)! African SNPs - African people have lots of SNP variation, even between each other! --> people originated in Africa...
View Full Document

  • Fall '12
  • HAFFIE
  • Biology, DNA, Mutation, DNA Damage, DNA Polymerase roofreading, DNA Damage vs, (mismatch distorts, cuts target sequence

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern