Bio 1001 Lecture 9 Summary

Bio 1001 Lecture 9 Summary - Bio 1001 Tom Haffie LECTURE 9...

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Bio 1001 Tom Haffie LECTURE 9 DNA Damage vs. Mutation Damage affects only one strand, mutations affects BOTH strands Repair mechanisms DNA Polymerase roofreading --> misaligned base , can't add next nucleotide, uses 3'-->5' exonucluease activity Mismatch repair: 1) repair enzyme scan (mismatch distorts shape); 2) Exonuclueases remove DNA chunk 3) DNA polymerase extends strand 5' --> 3'; 4) Ligase seals strands Mobile (Transposable) Elements - -> TE's --> mutations by transposing into genes 1. Insertion Sequence (IS) --> has inverted repeats on each side o "Cut and Paste" --> Transposase cuts backbones of target sequence o "Copy and Paste" --> DNA Poly copies & Transposase cuts target sequence 2. Transposon --> IS sequence on both ends --> move everything in between --> antibiotic resistance in bacteria
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Unformatted text preview: 3. Retrotransposons --> RNA polymerase (RNA intermediate, benefit?) --> Reverse Transcriptase--> Transposase Retroviruses Direct, not inverted repeat sequences + genes code for: o Gag --> proteins for RNA core; Pol --> reverse transcriptase and Intergrase ; env --> viral coat proteins Endogenous --> stuck in genome (chicken pox?)--> creates junk in genome o Called provirus after inserted into genome (HIV is a retro virus, Influenza isn't) Determining TE age 1. Amount of mobile element copies in genome! 2. Finding transposon in other species 3. Number of mutations it has between (other species and/or within same species/genome)! African SNPs - African people have lots of SNP variation, even between each other! --> people originated in Africa...
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  • Fall '12
  • Biology, DNA, Mutation, DNA Damage, DNA Polymerase roofreading, DNA Damage vs, (mismatch distorts, cuts target sequence

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