chp 5 group exercise - Chapter 5 Group Exercise Who is...

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Unformatted text preview: Chapter 5 Group Exercise Who is absent today? ‘1. Book problem 5.21: / 5.21 Shown in the accompanying gel are seven DNA frag- ments, designated A—G, present along the X chromosome of the laboratory rat Rattus norvrgiats. Each fragment was amplified by means of the polymerase chain reaction (PCR), as described in Chapter 6, using a unique pair of primers. The seven fragments are present in close proximity to each other along the X chromosome. Five small deletions in the X chromosome, designated A1 —A5. present in different strains, delete at least one of the templates for amplifica- tion. The gel shows the amplification products obtained from genomic DNA lrom males carrying each of the five deletions. Deduce the linear order of the amplified fragments A—G present in the wildtypc (undcleted) X chromosome. AlAZABMAS OfimUnw> team name: The two loci P and 82 are normally 36 m.u. apart on the same arm of a certain plant chromosome. A paracentric inversion spans about one—fourth of this region but does not include either of the loci. What approximate recom- binant frequency between P and 32 would you predict in plants that are . , - o a. Heterozygous for the paracentric inversion? = /L/ ' 36 » 2—7/4: 1). Homozygous for the paracontric inversion? L 36?. In com, the genes for tassel length (alleles T and t) and rust resistance (alleles R and r) are known to be on sep- a.“ {‘3‘ .t, r“ aratc chromosomes. In the course of making routine w_fi_ x I, crosses, a breeder noticed that one T/t ; R'/ r plant 4, I, + r' gave unusual results in a testcross with the double- recessive pollen parent t / t ; r / r. The results were Progeny: T/:;R/r 98 ~ ? tlt;r/r 104 ~ 7.7 ’ ’llvs pinhf gfvcsjlw‘ée 22».th buggy; 3.- T/ t ; rl r 3 m ; R / r s . Corn cobs: Only about half as many seeds as usual Eve “I. I We) Explain why the one exceptional plant is giving unusual results. a =Caac~rFo£ w, lrwéfo'sfocmlovo ‘7 \. 4. One of our Xavier neighbors is a young adult with Williams syndrome; l have often seen him in Gallagher Center and even met him a graduation party some years ago. Children with Williams syndrome are small, mentally retarded, and have highly characteristic “ellin” features. The disease often appears sporadically in families, but it can be inherited as an autosomal dominant disease. In a FISH analysis of patients' chromosomes, a cloned gene for the protein elastin is used as a probe. The probe hybridizes to only one homolog of chromosome 7 in 90% of cases. in the remainder of the cases (10%). the probe binds to both homologs. (if necessary, use your Reading Guide notes to remind your team about FISH.) From the choices below, choose m explanation for each tyge of Williams syndrome and indicate your choice by writing 90% or 10% by the appropriate explanation. 49: A. One homolog is heterozygous for the elastin gene. 10/, B. One homolog has an allele of the elastin gene that has a point (1-2 base) mutation. C. One homolog is the Y chromosome. . NJ; 3; . -. 1"“: "'0 " D. One homolog has a deletion of the region of the chromosome that would contain the elastin gene. 3 ~/‘ ‘x/ b \_/ h 5- A yellow body in Dmsophila is caused by a mutant 311- X , A A Progeny lele y of a gene located at the tip of the X chromosome Gray male 1 X yellow femalc , Females all yellow (the wild-type allele causes a gray body). In a radiation Males all gray experiment, a wild-type male W133 irradiated With X Gray male 2 X yellow female % females yellow rays and then. crossed with a yellow-bodied female. % females gray Most of the male progeny were yellow, as expected, but _ . 5 males yeuow the scanning of thousands of flies revealed two gray- - hum gray bodied (phenotypically wild-type) males. These gray- bodied males were crossed with yellow-bodied females, with the following results: i. r , ,1 w DNA}; Since radiation breaks chromosomes. it would be reasonable to suggest that the two exceptional flies have some sort of chromosomal rearrangement. Explain (show) how a translocation could explain the Explain (show) how a translocation could explain the results from gray male 1. results from gray male 2. \£:th01-/3405 Ill/ANS ibbgbrd ON ’2} JV KIM: W Herr“) 30.0”; fingmg 59,-; “I“. . 1i ‘ , _ 1, FL: ‘l l: b a “(I b D ‘j X ." x. K >1 X ’ x R K “j _ ‘ TQp,_-: to (of. furs) , ., '2 :cm 0 Kb \/ x Kg Xb ...
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  • Fall '12
  • DorothyB.Engle
  • Genetics

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