Lab 5: Quantifying Cold Tolerance of Various Species of Drosophila, and its Evolutionary Relationshi

Themeanwakeuptimeswouldthenbeanalyzedforrelationshipto

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Unformatted text preview: ionofwhetherornotthiscoldtolerance ­ orifitisduetothedevelopmentofsaidtoleranceinacommonancestor,thathasbeenslightly honedbyeachspeciesfortheirownneed.  Totestthis,16differentspeciesofDrosophilanativetodistinctregionsinrelationship totheequatorwereplacedinafreezerandthenremoved,andthetimeittookforthedifferent individualsofeachspeciestowakeupwascountedandcompliedtoformmeanwake  ­uptimes. Themeanwakeuptimeswouldthenbeanalyzedforrelationshiptothemidpointlatitudeof abitat.Furthermore,toestablishiftherelationshipisbasedonindependent naturalselectionofeachspecies,orifitwasbasedoncommonevolutionaryconnection,a correlationgraphwasalsotobegeneratedforcomparison MaterialsandMethods  Membersof16speciesofDrosophilawereplacedintubesbasedonspecies;these tubeswerethenplacedone ­by ­oneinthefreezerwith15 ­minuteintervalsinbetweenthem. Eachtubewasthenremovedaftercompleting3hoursofstayinfreezingconditions,inthe  orderofwhichtheycameout,sothateachtubewasremovedinintervalsof15minutes.Upon beingremovedfromthefreezer,themembers...
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