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Unformatted text preview: easuring these times in the crosses performed, statistical analysis would be done to
determine if there was a statistical difference between the distinct quantities of the different
crosses, and then based on the results of the previous experiment determining the identity of
it would be determined which variations, if any,
correlated to the infected status of the strains. MATERIALS AND METHODS
4 vials, color-coded Blue, Green, Orange, and White were obtained on Days 1 and 2. B
and G were males, O and W were female; B&W belonged to Strain A, while G&O belonged to
Strain B. For all performed crosses, the females were first placed inside clear tubes labeled with
the identity of the strains to be crosses using their respective colors. The male corresponding
with the desired cross was then placed inside with the female, where the tube was sealed with a
cotton ball and then observed to ensure copulation.
For Day 1, using a timer, the eight possible crosses 2 B&O crosses using two distinct
Blue and Orange N asoni a , 2 G&O, 2 B&W, and 2 G&W were each performed and observed,
and the time intervals between mounting, copulation, post-copulation remount, and dismou...
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