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Unformatted text preview: ss on the infection. Finally, in feminization, the Wolba chi a will override
the normal masculine development of the haploid males, and instead force them to become
haploid females and pseudo-females, which are often infertile. (Werren, 2003)
To confirm this, an experiment was conceived and performed where males and females
from two strains, A and B, were crossed, where one strain was Wolba chi a -positive and one was
Wolba chi a -negative. From the sex ratio distribution of offspring from the distinct crosses, the
identities of the distinct strains were to be identified, and confirmation of the identity of each
strain was to be provided by PCR amplification of a Wolba chi a-specific gene from a member of
each strain, followed by a standard gel electrophoresis analysis of the amplifications. Materials and Methods:
4 vials, color-coded Blue, Green, Orange, and White were obtained on Days 1 and 2. B
and G were males, O and W were female; B&W belonged to Strain A, while G&O belonged to
Strain B. For all performed crosses, the females were first placed inside clear tubes labeled with
the identity of the strains to be crosses using their respective colors. The male corresponding
with the desired cross was then placed inside with the female, where the tube was sealed with a
cotton ball and then observed to ensure copulation.
Eight crosses were performed with new individuals, but without the measuring of the
times; rather, the crosses were observed to ensure copulation, then the labeled and sealed tubes
were collected for later hosting for the eggs. The hosting involved the addition of two
Sa r cophaga fly pupae into each tube for the females to parasitize, and then the maintenance of
the tubes to ensure the proper development of the N asoni a within. After two weeks of
development, the partially-emerged wasps were collected and euthanized, and the sex ratios were
tallied. To determine the statistical significance of the data, a chi squared table of the results of
all the groups together was to be compiled using...
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This document was uploaded on 03/27/2014 for the course BIO 235 at Rochester.
- Spring '12