Bio 113P Lab 4: Effects of Wolbachia Infection on the Offspring Sex Ratio of Nasonia, vitripennis

In order for a difference to be considered

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Unformatted text preview: the statistics software, R. In order for a difference to be considered statistically significant, for the purposes of this study, a 95% confidence interval (R<.05) will be used as the cut-off point. The procedure used to perform and examine the PCR amplification was formerly described in WolbachiaandInsectBiodiversity:AstudyonthedegreeofWolbachiainfectionininsects andofthephylogenyrelationshipofthetwo(Vazquez1,2012). Results: The results of the crosses performed are shown below in Table 1; Figure 1 demonstrates graphically the distinct sex ratios observed per cross from the combined crosses from all the distinct groups. C r oss N umbe r of M a l es 22 N umbe r of F e m a l es 126 T ot a l Se x B r ood R a t io S i z e ( % M a l e) 148 14.9 127 4 131 96.9 24 211 235 10.2 37 148 185 20.0 Table 1 (above): Results from the distinct crosses Figure 1 (across): Graphical portrayl of general group sex ratios The combined results of the crosses across all groups are shown in Table 2, alongside the Chi-squared analysis: Cross BO BW GO GW NumberofMales 1443 1418 1015 2235 Numberof Females 2445 2843 2585 931 X2 1519.88 df p 3 <2.2e ­16  Table2:Resultsfromallcrossesperformed 2 analysisofthedifferencebetween themalesandfemales. Attached to the report is the image of the resulting gel plate from the gel electrophoresis (Figure 2); the rows from left to right are as follows: DNA Ladder, Wolba ch i a -positive control, Wolba chi a -negative control, Strain A, Strain B, Water (DNA-negative control). Discussion: As can be seen in Table 1, the most dramatic ratio can be seen in the second cross, between Male A and Female B, wherein we see not only a 96.9% male population, but also that the group has the lowest total brood size. This is characteristic of CI, which as previously mentioned, is indicative of an infected male mating with an uninfected female. In contrast,...
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This document was uploaded on 03/27/2014 for the course BIO 235 at Rochester.

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