Enzyme Lab Report - Lab Report By Joshua Eusebio 2.26.14...

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Lab Report By: Joshua Eusebio 2.26.14 Tuesday Night Lab Hasse
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Introduction: In this lab we focused our attention on enzymes and denaturation. Enzymes in a very broad sense are proteins that act as catalysts, speeding up chemical reactions in cells. We have roughly 4,000 enzymes in our body that we know the function of. Enzymes are extremely important to us because they are responsible for speeding up reactions that synthesize and degrade materials that are basic building blocks for our body. They also play a role in creating energy, which is extremely important. Denaturation is the alteration of a protein shape through some type of external force in such a way that it is no longer able to carry out its cellular function. In some instances proteins can regenerate with the removal of the denaturing agent and restoration of conditions that the protein favors. In our experiment we used Jell-O, which is made up four basic ingredients: gelatin, water, sugar, and artificial flavors. Jell-O is particularly interesting because of the gelatin in it. Gelatin is a processed version of collagen, which is a big fibrous molecule that makes skin, bones, both strong and somewhat elastic. Pineapple is used in this experiment because of what it is capable of doing to gelatin. Fresh pineapple produces a proteolytic enzyme called bromelain that digests gelatin. This disallows the bonds from being formed in Jell-O, so the solution never becomes solid. Hypothesis: If a change in the pH can denature an enzyme and its function, then soap, which is a base, should denature the enzyme pineapple called bromeliad in a gelatin solution. Materials: Jell-O, Test tubes, Ice, Water, Fresh Pineapple, Canned Pineapple, Pipettes, Timer, Test tube racks, Balance, 250 mL beaker, tweezers, soap, knife
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Procedure: Collect all materials and label the test tubes. Cut four (4) cubes of FRESH pineapple weighing precisely 2 grams each. Cut one (1) cub of CANNED
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