Protist Lab Report - Josh Eusebio Protist Lab Report...

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Josh Eusebio Protist Lab Report Introduction: In our experiment we studied the effects that Daphnia (a small planktonic crustacean that feeds on bacteria, algae, and yeasts) would have on the populations of Paramecium caudatum and Paramecium bursaria if they were placed in the same wells . Paramecium caudatum is a unicellular organism belonging to the genus Paramecium of the phylum Ciliophora. They feed on bacteria and small eukaryotic cells (any organism whose cells contain a nucleus and other structures enclosed within membranes). Paramecium bursaria is a species of ciliate protozoan algae called Zoochlorella. They have a symbiotic relationship with algae where the alga provides them with food and the Paramecium bursaria provides the algae with movement and protection. From what we have learned in class, we have proposed that the P. bursaria would be more preyed upon than the P. caudatum by the Daphnia. If P. bursaria, P. caudatum, and Daphnia are all placed in the same well, and the P. bursaria are slower and have a less direct pattern of movement, then the P. bursaria will be more preyed upon by the Daphnia compared to the P. caudatum. Materials : Stereoscopic binocular microscope Protist cultures Volumetric pipettes and dispensers Pasteurized spring water Concentrated liquid food Pasteur pipettes and bulbs Depression slides 6-well culture plates Procedure : Procedure for Lab 1 followed with the following modifications : 1. Instead of just adding P. bursaria to the first three wells and P. caudatum to the next three wells, we added P. bursaria and Daphnia into the first three wells, followed by adding P. caudatum and Daphnia to the next three wells. These 6 wells will be our control variables . In the last three wells a mix of P. caudatum, P. bursaria , and Daphnia was added to each. This was our experimental variable . Results: 1
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Graph 1 D a y 0 5 7 9 1 2 1 3 P . C . 1 0 2 1 1 3 7 2 3 2 3 3 5 0 0 1 0 2 3 P . C . 2 1 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0 P .
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  • Fall '13
  • SaraChoung
  • Chemistry, pH, P. caudatum, Paramecium caudatum, P. bursaria, Paramecium bursaria

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