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Unformatted text preview: Open conformation is further stabilized by membrane lipid interaction Coincidence detection and
AP2 binds cytosolic cargo sorting
motif YxxØ at PM but not Golgi
Additional AP-2 binding site provided
by PtdIns(4,5)P2 at PM reinforces
fidelity of cargo sorting process Self-assembly
& stimulation of the GTPase activity Dynamin (shibire) Binds phospholipids
(PIP2) and membranes Binding to endocytic
components Lipid coated coated microspheres
incubated with GTP and dynamin
leads to vesicle formation in vitro Dynamin is a mechanochemical enzyme and drives membrane fission,
but most likely has additional functions through multiple interactions with
proteins involved in early and late steps of clathrin dependent endocytosis Temperature sensitive dynamin mutant shibire induces paralysis in fly
at restrictive temperature due to defective synaptic vesicle endocytosis
and recycling. Shibire WT Temporal assembly of components during clathrin-mediated
endocytosis Endocytic routes
Summary (Sorting endosomes) (MVBs)...
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This note was uploaded on 03/27/2014 for the course BCH 444 taught by Professor Mccallan during the Spring '14 term at University of Toronto- Toronto.
- Spring '14