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To allow cargo binding via 2 open conformation is

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Unformatted text preview: Open conformation is further stabilized by membrane lipid interaction Coincidence detection and Cargo selection: AP2 binds cytosolic cargo sorting motif YxxØ at PM but not Golgi Additional AP-2 binding site provided by PtdIns(4,5)P2 at PM reinforces fidelity of cargo sorting process Self-assembly & stimulation of the GTPase activity Dynamin (shibire) Binds phospholipids (PIP2) and membranes Binding to endocytic components Lipid coated coated microspheres incubated with GTP and dynamin leads to vesicle formation in vitro Dynamin is a mechanochemical enzyme and drives membrane fission, but most likely has additional functions through multiple interactions with proteins involved in early and late steps of clathrin dependent endocytosis Temperature sensitive dynamin mutant shibire induces paralysis in fly at restrictive temperature due to defective synaptic vesicle endocytosis and recycling. Shibire WT Temporal assembly of components during clathrin-mediated endocytosis Endocytic routes Summary (Sorting endosomes) (MVBs)...
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