• Targeting nascent proteins to the endoplasmic reticulum• Protein translocation into the ER, membrane protein topology• Protein glycosylation, functions of glycoproteins• Protein folding in the ER, role of chaperones and folding catalysts • Protein quality control - ERAD and the UPRDavid B. Williams, Room 5316 MSB [email protected]BCH 444 Spring 2014 1
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2 Endoplasmic Reticulum (ER) - first stage in the secretory pathway - About 30% of cellular proteins are synthesized on membrane-bound ribosomes of the ER and are translocated partially (membrane proteins) or completely (soluble proteins) across the ER membrane. - This segregates them from other cellular proteins and establishes their topology as transmembrane or soluble proteins - These proteins are subsequently sorted to their final destinations as they move along the secretory pathway from ER to Golgi to cell surface