exam 4 Fall 2010 - #$'$ $%$#$/01 23#4!...

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kJ/(mole K); T = 298 K; F = 96.485 kJ/(V mole); F = 1 eV/V mole S'3/1,-'0 -L H 3$'-11 3 %#%&'3/# L'-% 1.K# ( 0- 1.K# *; # Q : RSM/ UHV * WUHV ( 7 _ F #$ #$ = $ 2 % $ 1 3/K _ .1 0^# $^3'`# -/ H; 1 mole is 6.023 X 10 23 items; *P700 is about 1.8 eV above the ground state a : ^$W & > ^ : PM3/$\91 $-/103/0 : O;O*O b (+ 8E< ] 1#$ $ : 1,##K -L M.`^0 ./ 3 N3$JJ% : E b (+ @ %W1#$> & : c3N#M#/`0^ -L M.`^0 ./ %#0#'1; H %-M# -L ,^-0-/1 .1 O;+*E b (+ *E -L 0^#%> ( $3M-'.# .1 <;(@ ] Multiple Choice: (5 points each) 1) When halophilic archeabacteria are exposed to light under conditions of low oxygen so that photophosphorylation kicks in, the external pH a) goes down b) goes up c) get real, teacher, they can’t change the pH of their pond d) none of the above 2) In vision, the phosphodiesterase (PDE) enzyme activity is found a) inside the disc space b) in the cell cytoplasm c) in the nucleus of the cell d) in the synapse between two cells
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2 3) k cat /K M is an effective second order rate constant which means it is a) diffusion limited in water at room temp to between 10 9 and 10 8 M -1 sec -1 b) limited by nuclear motion to about 10 13 s -1 c) not limited by any physical processes d) subject to price controls 4) ATP synthase make 3 ATP per turn. If # G for making ATP is ~50 kJ/mole, the minimum number of protons required to flow through the ATPase per turn would be (assume a fully energized mitochondrical membrane so that the free energy for moving a charge across it is ~19 kJ/mole).
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