BCH463_Crystallography - Green Fluorescent Protein Color...

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Green Fluorescent Protein: Color Variation and Chromophore Formation
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Sets of parallel lines that pass through a two-dimensional lattice
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Orthorhombic Direct and reciprocal cells Monoclinic Triclinic
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Protein molecules in a crystal (with unit cell)
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Separation of optical image formation into two stages, and comparison of optics to x-ray diffraction
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Diffraction pattern collected on a protein crystal
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Diffraction pattern of a salt crystal
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Fourier synthesis of a periodic function from a sum of waves with periods 1/n
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Fourier synthesis of electron density in a one-dimensional crystal of a diatomic molecule
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YFP-H148Q
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Unformatted text preview: crystal soaked in KI Anomalous difference map identifies two bound iodides Information used for refinement Yarbrough, Wachter, Kallio, Matz, Remington (2001), PNAS 98 , 462-467. Part of a refined protein model (DsRed, a red-fluorescent GFP-like protein) Electron density map of YFP T203Y/S65G/ V68L/S72A p-stacking of Tyr203 with chromophore plane-to-plane distance 3.3 to 3.8 A T203Y (~15 nm) S65G (~ 5 nm) Chromophore Wachter, Elsliger, Kallio, Hanson, Remington (1998), Structure 6 , R1267-1277. Extra Slides Example of a common unit cell Experimental Map (using experimental phases, no refinement)...
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  • Spring '14
  • Atom, Crystal system, Green fluorescent protein, Wachter

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