MBB331_Week10-Fall-2011

Factor families transcription homeodomainmotifs helix

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Unformatted text preview: homeodomain of Drosophila Drosophila Engrailed bound to DNA determined determined Lewin Genes VII 2000 Oxford University Press 7 TRANSCRIPTION FACTOR FAMILIES: TRANSCRIPTION HOMEODOMAIN MOTIFS • Helix-3 lies in major groove and Helix-3 makes majority of contacts with DNA, helix-1 projects into minor groove and makes other contacts with DNA contacts Lewin Genes VII 2000 Oxford University Press 8 TRANSCRIPTION FACTOR FAMILIES: HELIX­LOOP­ HELIX­ HELIX (HLH) MOTIFS • • • • • Helix-loop-helix motif: 40-50 amino acids - 2 amphipathic α helices separated by loop HLH amphipathic helices: one HLH helix face hydrophobic, other face charged & hydrophilic HLH proteins form both HLH homodimers and heterodimers through interactions between hydrophobic amino acids in helices helices bHLH proteins have highly basic HLH DNA binding region adjacent to HLH motif HLH a homodimer or heterodimer in homodimer which both subunits contain basic both region can bind DNA like a pair of tongs tongs Weaver 2002 Molecular Biology Ed. 2 9 TRANSCRIPTION FACTOR FAMILIES: HELIX­LOOP­HELIX (HLH) M...
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This note was uploaded on 03/26/2014 for the course MBB 321 taught by Professor Davidson during the Spring '11 term at Simon Fraser.

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