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Falciparum during its life cycle var facilitates long

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Unformatted text preview: ce of retinoic acid, retinoic acid receptor (RAR) & retinoic acid receptor X (RXR) heterodimer binds sites that repress transcription N-CoR/SMRT (nuclear receptor co-repressor/silencing mediator for retinoid & thyroid hormone receptors) bind histone deacetylase HDAC1 34 35 ChIP can be used to elucidate “histone code” of single genes or whole genomes • ChIP using antibodies against methylated and acetylated histones can reveal the histone code of single genes or whole genomes under particular conditions • For example, ChIP was used to look at the histone code of upstream control region of the var gene of the malaria parasite, P. falciparum, during its life cycle • var facilitates long-term chronic infection of the host 36 37 38 REGULATING TRANSCRIPTION BY AFFECTING CHROMATIN STRUCTURE • Global changes in chromatin structure can regulate transcription: • eg. yeast SWI/SNF “chromatin remodeling” favours transcription by altering nucleosome density/structure • Drosophila Polycomb proteins repress homeotic genes by affecting chromatin structure - polycomb null mutants cause homeotic gene expression in incorrect locations 39 40 (2) LONG RANGE TRANCRIPTION REGULATION: (2) β -GLOBIN GENE CLUSTER Lewin Genes VII 2000 Oxford University Lewin Press Press • • • • • Transcriptional regulation = promoters + enhancers + locus control Transcriptional regions (LCRs) for some genes regions β -globin gene cluster: distant DNase I hypersensitive s...
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