MBB331_Week10-Fall-2011

Falciparum during its life cycle var facilitates long

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: ce of retinoic acid, retinoic acid receptor (RAR) & retinoic acid receptor X (RXR) heterodimer binds sites that repress transcription N-CoR/SMRT (nuclear receptor co-repressor/silencing mediator for retinoid & thyroid hormone receptors) bind histone deacetylase HDAC1 34 35 ChIP can be used to elucidate “histone code” of single genes or whole genomes • ChIP using antibodies against methylated and acetylated histones can reveal the histone code of single genes or whole genomes under particular conditions • For example, ChIP was used to look at the histone code of upstream control region of the var gene of the malaria parasite, P. falciparum, during its life cycle • var facilitates long-term chronic infection of the host 36 37 38 REGULATING TRANSCRIPTION BY AFFECTING CHROMATIN STRUCTURE • Global changes in chromatin structure can regulate transcription: • eg. yeast SWI/SNF “chromatin remodeling” favours transcription by altering nucleosome density/structure • Drosophila Polycomb proteins repress homeotic genes by affecting chromatin structure - polycomb null mutants cause homeotic gene expression in incorrect locations 39 40 (2) LONG RANGE TRANCRIPTION REGULATION: (2) β -GLOBIN GENE CLUSTER Lewin Genes VII 2000 Oxford University Lewin Press Press • • • • • Transcriptional regulation = promoters + enhancers + locus control Transcriptional regions (LCRs) for some genes regions β -globin gene cluster: distant DNase I hypersensitive s...
View Full Document

This note was uploaded on 03/26/2014 for the course MBB 321 taught by Professor Davidson during the Spring '11 term at Simon Fraser.

Ask a homework question - tutors are online