MBB331_Week10-Fall-2011

Intron side lewin genes vii 2000 oxford university

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Unformatted text preview: N • Cleavage/ligation of Cleavage/ligation exons occurs not as separate events but through a single coordinated process coordinated • Transesterification: 2´-OH 2´-OH of invariant “A” in branch site attacks 5´ splice site site • Release of exon 3´-OH Release end attacks 3´splice site & second transesterification excises intron excises Weaver 2002 Molecular Biology Ed. 2 73 THE SPLICEOSOME THE SPLICEOSOME • Spliceosome: sequentially assembled complex of small Spliceosome: nuclear RNAs (snRNAs) with proteins that together form ribonucleoprotein particle (snRNPs) ribonucleoprotein • Yeast snRNAs: all 5 snRNA genes in yeast are essential Yeast for viability - mutations in snRNA block splicing for • snRNPs (named after snRNAs they contain): – U1, U2, U5, and U4/6 RNAs – together contain ~40 different proteins, but all have together common structural core of 8 proteins and all recognized by anti-Sm autoimmune antiserum recognized 2 BROAD STAGES OF SPLICING: BROAD -recognition of consensus sequences and complex assembly assembly -cleavage/ligation reactions 74 U1 snRNP U1 snRNP • U1 sn...
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This note was uploaded on 03/26/2014 for the course MBB 321 taught by Professor Davidson during the Spring '11 term at Simon Fraser.

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