MBB331_Week10-Fall-2011

With 4 subunits with rna binding motifs rna binding

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Unformatted text preview: e, at position N6 at 57 Weaver 2002 Molecular Biology Ed. 2 CAP STRUCTURES CAP STRUCTURES AT 5´ mRNA ENDS -in some species, a methyl group is added -in to the 2´-O (ribose) position of the 3rd base, creating the cap 2 type cap -cap may provide mRNA stability and aids -cap in translation initiation as it is recognized and bound by the 40S ribosome subunit and -cap may also be used in splicing out of -cap introns introns 58 Weaver 2002 Molecular Biology Ed. 2 POLYADENYLATION AT 3´ mRNA ENDS POLYADENYLATION • Unlike in bacteria, RNAP II Unlike stops transcription at multiple “termination regions” regions” • Precise 3´ end of transcript Precise defined by mRNA cleavage & polyadenylation polyadenylation • During synthesis, RNAP II During transcribes past site used to make 3´end: to – AAUAAA site, ~11-30bp AAUAAA upstream of site where poly(A) added- common feature of mRNAs in higher eukaryotes but not yeast not • AAUAAA recognized by AAUAAA specificity component specificity (CPSF) with 4 subunits with RNA-binding motifs RNA-binding Lewin Genes VII 2000 Oxford University Press 59...
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This note was uploaded on 03/26/2014 for the course MBB 321 taught by Professor Davidson during the Spring '11 term at Simon Fraser.

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