Ch. 10 Review - Recombinant DNA technology genetic...

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Recombinant DNA technology: genetic engineering Restriction nucleases- cut DNA molecules at specific sites, are produced by various bacteria o Only 5-8 nucleotides long o Reliably generates the same DNA fragments Gel electrophoresis- separates DNA fragments into different sizes o A voltage is applied across the fragments and they separate based on ionization and size o Visualized using fluorescent dyes or radioisotopes Southern blotting- allows the search for nucleic acids with a complementary sequence on a DNA probe o The probes contain a fluorescent or radioactive label to facilitate the detection of the nucleotide sequence with which they bind. Can be synthesized because we have so much information about where and what genes are present in the cells. DNA hybridization = DNA renaturation after the strands have been taken apart with heat o Allows us to identify specific nucleic acid sequences in the DNA DNA cloning= production of many of the same DNA molecules PCR = polymerase chain reaction allows a direct approach to cloning that allows copying of one copy in a test tube o Recombinant DNA molecules are DNA strands formed from fragments attached by DNA ligase o A vector is a carrier DNA molecule for DNA fragments allows amplification of the fragment; the vector in this case is plasmids which are
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