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E2 F12 answer key - Che 4342 Topics in Human Biochemistry...

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Che 4342: Topics in Human Biochemistry EXAM 2 10/16/12 Name:_________________________ Choose the single best answer. Only your scantron will be graded. 1-30, 3.5 pts each 1. In general, how is the melting curve of duplex DNA in an aqueous solution affected by addition of a small amount of ethanol? a. the T m would increase b. the T m would decrease c. the T m would stay approximately the same d. it would depend on the G-C content of the DNA e. further information is required to answer this question 2. How does the D-ribose pucker affect DNA structure? a. the ribose pucker dictates the relative orientation of the phosphate backbone group to the carbohydrate in each residue b. in B DNA it is C2 / - exo c. in B DNA it is C3 / - endo d. in A DNA it is C3 / - exo e. in A DNA it is C5 / - exo 3. In addition to the standard base-paired helical structures, DNA can form specialized structures. What type of structure often occurs at sequences with inverted repeats such as TCAAGTCCACGGTGGACTTGC? 4. Which of the following statements about nucleosomes is NOT correct?
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2 5. Which of the following statements about topoisomerases is NOT correct? 6. Examine the Figure of the eukaryotic replisome below. Which of the following statements about proteins that participate in mammalian DNA replication is NOT correct? a. A designates one subunit of the ATP-dependent helicase, MCM b. B designates DNA pol ε , a classical B family enzyme with proofreading 3 / –>5 / exonuclease activity c. C designates RFC, replication factor C which is the ATP-dependent clamp loader d. D designates DNA pol α e. E designates RPA, replication protein A which binds to single stranded DNA and prevents it from re-annealing
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