E2 F12 answer key

Name 31 34 50 pts total including

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Unformatted text preview: _____________________ (31-34): 50 pts TOTAL including 5 pts extra credit) 31. 15 pts A. With chemical structures, draw the chemical reaction for the incorporation of the next (one) nucleotide into the DNA molecule below. Show the chemical structure of the DNA product (last 2 nucleotides). (Show the complete chemical structure of the incoming substrate that is used for this reaction and products, but only the terminal nucleotide of the DNA substrate needs to be shown. You do NOT need to draw the complete DNA template strand, but draw the individual bases with H-bonds between the two strands. Once a base has been drawn, it may be abbreviated. However, be sure to draw all structure first (including phosphates for example). 3’-TACGCGCATTCACAT---5’ 5’-ATGCGCGTAA-3’ DNA Polymerase Reaction 5'-(dNMP)n- 3'-OH + dNTP !5'-(dNMP)n-dNMP -3'-OH + PPi NH2 N N O O- P O CH2 N OH O H H H O H P O– P O O– O N CH2 H O– H –O dGTP H H OH O NH2 N O H Inorganic pyrophosphatase NH O P O C N 5/ –O T O H 3/ O– O N O P O P O– PPi O– OH Mg2+ NH2 N N O O- P O CH2 H O– N O O H 3/ H new phosphodiester bond H 5/ P O N CH2 H O O 3/ c N H O –O T N NH N NH2 H H H OH H 10 B. What is the role of the two Mg2+ ions in the reaction mechanis...
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This test prep was uploaded on 03/23/2014 for the course CHEMISTRY 4141 taught by Professor Jonklaas during the Spring '13 term at Baylor.

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