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Unformatted text preview: e RNA substrate
needs to be shown. You do NOT need to draw any of the DNA strand.)
O- N P O O
H 3/ H H
–O O P P O N 5/ O O– H O– H OH H
O PPi –O O O OH O– A NH OH O P O N O
H O– T N H O P P O O– OH O– Mg+2 T
O- P A O O
H O– H 3/ H new phosphodiester bond
–O P A H
O O OH 5/ U
H O 3/ H H H
OH OH 14 B.
What is the general name of the enzyme that catalyzes this reaction?
RNA polymerase II
How are specificity and transcriptional fidelity achieved in the enzymecatalyzed reaction?
1. ~ equal concentrations of nucleotide triphosphate substrates in pool
2. 2-stage RNA pol II reaction which tightens the active site to facilitate the reaction when
there is good base pairing with the incoming NTP and the template DNA at the addition site
3. RNAP II with its ratchet type movement can back up and cleave mismatched DNA/RNA
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This test prep was uploaded on 03/23/2014 for the course CHEMISTRY 4141 taught by Professor Jonklaas during the Spring '13 term at Baylor.
- Spring '13