E2 F12 answer key

What is the role of the two mg2 ions in the reaction

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Unformatted text preview: m? Mg2+ ion A activates the “primer” 3/-OH for nucleophilic attack on the alpha phosphorus (and also helps to orient it and electrostatically stabilize it) while Mg2+ ion B orients the triphosphate group and electrostatically stabilizes it C. Describe the ways in which the fidelity of the reaction is ensured. ~ equal concentrations of deoxynucleotide triphosphate substrates in pool 2-stage DNA pol reaction which tightens the active site to facilitate the reaction when there is good base pairing with the incoming dNTP and the template 3/ -> 5/exonuclease activity with DNA pol δ and ε primer requirement DNA repair mechanisms especially MMR & BER 11 Initials:___________________ 32. 10 pts A. XPA, RPA, XPC-TRIIH, XPB (a 3’->5’ helicase) & XPD (a 5’->3’ helicase) of TFIIH, XPG, XPC, XPF(a 5’-endonuclease), XPG (a 3’-endonuclease), DNA pol delta or epsilon , PCNA, DNA-ligase are all important proteins in which DNA repair system? Nucleotide Excision Repair (NER) B. Describe this system...
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This test prep was uploaded on 03/23/2014 for the course CHEMISTRY 4141 taught by Professor Jonklaas during the Spring '13 term at Baylor.

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