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.030 Absorbance Absorbance vs. Concentration
y = 0.0004385x + 0.0125865
R2 = 0.9910977 0.4
Linear (Series1) 0 200 400 600 800 1000 Concentration (μg/mL) The first step in analyzing the results was to determine the standard curve for standard
solutions of bovine serum albumin by plotting a graph of absorbance vs. concentration. The data
point with the highest concentration of protein was disregarded because it was an outlier that did
not fit the rest of the data. The inclusion of that point suggested an almost logarithmic curve. The
linear graph without that data point, then, had a R2 value of .9911, which was relatively good
because it indicated that most of the points fell relatively close to the best fit line drawn.
By using the standard curve, the unknown concentrations were found by finding the
concentration corresponding to the absorbance measured, and then multiplying by the dilution
factor to find the actual concentration of the unknown. The four results averaged out to be about
8038 μg/mL, or 8.038 mg/mL, with a standard deviation of 8038 μg/mL ± 1080 μg/mL. Calculations:
Using the equation of the standard curve and graphical analysis:
A1: Y = 0.0004385x + 0.0125865 .323 = 0.0004385x + 0.0125865
x = 707.8 μg/mL
Since it is a 10x dilution,...
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- Spring '13