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Unformatted text preview: or that point. This concentration can then be multiplied by the dilution factor to
find the concentration of the original unknown sample. Also the response of the dye to protein is
linear only for a certain range of a protein’s concentration.8, 9 Experimental:
All chemicals, including both the standard solution of bovine serum albumin and the
solution of bovine serum albumin of unknown concentration, were obtained from Sigma (St.
Louis, Mo) unless otherwise noted. The spectrophotometer used was a DU 520 Beckman (Fullerton, CA) General Purpose UV/Vis Spectrophotometer. The water used was deionized
water from the Baylor Science Building (BSB Waco, TX).
Serial dilutions of the stock standard solution of bovine serum albumin (St. Louis, Mo),
or BSA, were made using deionized water (BSB Waco, TX) and the stock standard solution to
give six samples of standard solutions ranging from concentrations of 68 μg/mL to 1428 μg/mL
in six test tubes, according to the dilution table in the results section. The test tubes were then
covered with parafilm to prevent evaporation.
Next, a 10x dilution was made of the unknown BSA by adding 900 μL of deionized
water to 100 μL of unknown BSA solution to form sample “unkn. A1.” This process was
repeated to make sample “unkn. B1” in a separate test tube. 5x serial dilutions of “unkn. A2” and
“unkn. B2” were then made from “unkn. A1” and “unkn. B1,” respectively, by adding 400 μL of
deionized water to 100 μL o...
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This note was uploaded on 03/23/2014 for the course CHEMISTRY 4141 taught by Professor Jonklaas during the Spring '13 term at Baylor.
- Spring '13