Intact pcon6 is about 8000 bp and has 1 ecori site

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Unformatted text preview: Roughly how far apart are the EcoRI and HindIII sites? A. 1,000 bp B. 1,900 bp. C. 2,500 bp. D. 3,600 bp. E. Can’t tell from the gel. iClicker Question 6: Which restriction site is closest to the EcoRI site? A. ClaI B. HindIII C. NruI D. PstI E. Can’t tell from the gel iClicker Question 7: You want to PCR the thick region of the DNA below. Which primer pair do you use? A B 5’ 3’ 3’ 5’ C A. B. C. D. E. A and B C and D A and D B and C None of the above D iClicker Question 8: The ClaI site below is only present in the A allele. Suppose you PCR this region from a person with genotype Aa and digest with ClaI. What bands would you see? ClaI 5’ 3’ 3’ 5’ A. B. C. D. E. 600 bp 600 bp 300 bp 600, 400, and 200 bp 600 and 300 bp 400 and 200 bp...
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This note was uploaded on 03/28/2014 for the course BIO 111 taught by Professor White during the Fall '11 term at University of Massachusetts Boston.

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