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Unformatted text preview: be part of pGLO? A. So that pGLO will be copied in the bacterium. B. So that you can select for cells carrying pGLO. C. So that the cells will produce GFP. D. So that pGLO can replicate in human cells. E. None of the above. iClicker Question 6: A close- up of the AflII site in pGLO is on the handout. Which of the other enzymes listed will cut at this site? A. MseI B. Tsp509I C. HpaI D. More than one of the above. E. None of the above. iClicker Question 7: Suppose you digested pGLO with AgeI and XmaI, what length fragments would be produced? A. 1,074 and 2,079 bp B. 5,371 and 1,005 bp C. 5,371 bp only D. 1,005 bp only E. 4,366 and 1,005 bp iClicker Question 8: Suppose you wanted to make a translational fusion protein: N-{beta-globin}-{GFP}-C (This is one big “franken- protein” that starts with the amino acids for beta- globin and continues through GFP without stopping until the C- terminus of GFP) At which restriction site would you insert the coding region for beta- globin (with its stop codon removed)? A. NheI (cuts @ 1345) B. BstBI (cuts @ 1966) C. EcoRI (cuts @ 2063) D. ScaI (cuts @ 2942) E. AlwNI (cuts @ 4339)...
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This note was uploaded on 03/28/2014 for the course BIO 111 taught by Professor White during the Fall '11 term at University of Massachusetts Boston.

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