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Unformatted text preview: wer in rough and then transfer it to the exam paper. (b) Based on ∆HH-bond alone, should the protein fold in water? ___________ (YES/NO) 10 Question #10 (TUTORIAL content, 10 points)
(a) In an effort to purify histone proteins from nuclear extracts, a biochemist applies a sample of nuclear
proteins to a column containing a matrix composed of cellulose derivatized with DNA Following application
of the sample in buffer at pH = 7, the column is washed with an NaCl gradient in buffer at pH = 7.
What kind(s) of column chromatography is the biochemist using?
( iv) Cation exchange chromatography
Anion exchange chromatography
Size exclusion chromatography (b) You are investigating the properties of three monomeric proteins A, B and C, and find:
When a mixture of the three proteins is subjected to native polyacrylamide gel electrophoresis, protein C lies
nearest the bottom of the gel followed by A, and finally B which lies nearest the top of the gel (the point
of sample application)
Protein A binds to diethylaminoethylcellulose at pH 5.5 but not at pH 4.5.
In 2-D gel electrophoresis, Protein B ceases migration when it reaches a pH = 6.0 in the1st dimension.
Protein C is displaced from carboxymethylcellulose by Na+ at pH = 6.
Using this information, answer the f...
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- Winter '13