Practice MT

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Unformatted text preview: wer in rough and then transfer it to the exam paper. (b) Based on ∆HH-bond alone, should the protein fold in water? ___________ (YES/NO) 10 Question #10 (TUTORIAL content, 10 points) (a) In an effort to purify histone proteins from nuclear extracts, a biochemist applies a sample of nuclear proteins to a column containing a matrix composed of cellulose derivatized with DNA Following application of the sample in buffer at pH = 7, the column is washed with an NaCl gradient in buffer at pH = 7. What kind(s) of column chromatography is the biochemist using? ( i) ( ii) ( iii) ( iv) Cation exchange chromatography Anion exchange chromatography Affinity chromatography Size exclusion chromatography (b) You are investigating the properties of three monomeric proteins A, B and C, and find: When a mixture of the three proteins is subjected to native polyacrylamide gel electrophoresis, protein C lies nearest the bottom of the gel followed by A, and finally B which lies nearest the top of the gel (the point of sample application) Protein A binds to diethylaminoethylcellulose at pH 5.5 but not at pH 4.5. In 2-D gel electrophoresis, Protein B ceases migration when it reaches a pH = 6.0 in the1st dimension. Protein C is displaced from carboxymethylcellulose by Na+ at pH = 6. Using this information, answer the f...
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