201-13-5-Metabolism II-April 3 & 5-2 to post

Detailed mechanisms of pdh are not testable material

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Unformatted text preview: porters including ones for Pyruvate Succinate Monocarboxylates (eg. β- hydroxybutryate) & other compounds No transporters for NADH Malate-Aspartate Shuttle! Animal Metabolism (II)! Under aerobic condi,ons, how is NAD+ regenerated in the cytoplasm to support con,nued func,oning of glycolysis? How does NADH get into the mitochondria? Animal Metabolism (II)! The Pyruvate Dehydrogenase Complex! Animal Metabolism (II)! The Pyruvate Dehydrogenase Complex! Pyruvate (from Cytosol) Transported Mitochondria NAD+ Ketone Reduced to NADH Oxidized to Thioester Animal Metabolism (II)! The Pyruvate Dehydrogenase Complex! Role of the vitamin Thiamine (B1) in PDH Reac,on. Thiamine is part of prosthe,c group Thiamine Pyrophosphate (TPP) TPP is bound in the ac,ve site of PDH Covalent bond is formed between carbanion and TPP Role of Thiamine in TPP is to spread out the –ve charge on the Carbanion Animal Metabolism (II)! The Pyruvate Dehydrogenase Complex! Detailed mechanisms of PDH are not testable material, but know how carbanion is stabilized See Moran et al. p 329 to 331 Animal Metabolism (II)! The Pyruvate Dehydrogenase Complex! NAD+ CoA-SH Pyruvate Ca2+, NAD+, CoA-SH, H+ NADH CO2 Acetyl-CoA PDHa NADH, ATP, Acetyl-CoA High Activity PDH kinase PDH phosphatase NADH, Acetyl-CoA ADPfree, Ca2+, NAD+, Pyr., CoA-SH P PDHb P Low Activity Animal Metabolism (II)! Tricarboxylic Acid Cycle! Products of interest...
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