5-Enzyme mechamism handout2

Intermediate formed moreresonance contributors

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: -leavinggroup withcatalysis,wehaveROH Goodnucleophile,butbadleaving group,viceversa mostimportantstepis.. ionpair:stabilize hydrogenbondto certaindegree RNase: acid/base catalysis 3. Acid forms a better leaving group (ROH) Transition State #1 uncatalyzedsituation goodleaving group,process isnowfast badleaving group e- migration - Leaving group (hasn’t left yet) e- migration O ---------- P ----------- O-5’-Ribose Bond forming Bond breaking Development of negative charge RNase: acid/base catalysis 3. Acid forms a better leaving group (ROH) a) His-119 acts as a base/acid and donates a proton to the 5’-oxygen atom of the next nucleotide residue to produce an alcohol leaving group – Product 1 (P1). RNase: acid/base catalysis 4. Intermediate formed RNase: acid/base catalysis 4. Intermediate formed moreresonance contributors (chargeargument weaker) tetrahedral...
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern