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5-Enzyme mechamism handout2

Note that water is excluded from the active site due

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Unformatted text preview: 2’-oxygen atom, leading to a better leaving group, Product 2 (P2) – a 3’-nucleoside monophosphate Overview 1. Introduction: Thermodynamics vs. kinetics Catalysis Enzyme catalysis 2. RNase: (Example 1) Function, structure, reaction Mechanism without catalysis Transition state stabilization Acid/base catalysis Specificity 3. Chymotrypsin: (Example 2) Reaction cycle Covalent catalysis Acid/base catalysis Transition state stabilization RNase: Transition state stabilization TS stabilized by binding to active site residues RNase: Transition state stabilization Further stabilization through charge neutralization TS1 ts2 couldsametypeofHbondhappeninintermediate,startingmaterial,products?->products:crowding, spacerequirements,pushesoxygenfurtherapart,stabiliziestransitionstatewithoutloweringEScomplex exact...
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