5-Enzyme mechamism handout3

Enzymatic rnasecatalyzed substrate product 2

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Unformatted text preview: 2cannotdeprotonatethe2'OHofDNA,nonucleophile startreaction RNase: specificity (Most) Enzymes are highly selective catalysts 3. Particular reaction steps For example in the RNase mechanism: Location of His-12 relative to the atoms of the cyclic intermediate ensures that only 3’ phosphate (but not 2’) products formed. compareandcontrast: betweennon-enzymaticalkalinehydrolysisofRNAandRNasemediaterdenzymaticRNAhydrolysis: alkalinehydrolysis:hydrolyzeRNAintesttubeathighpH,mechanismsimilartobutnotidenticalto enzymatichydrolysis NON-enzymatic alkaline hydrolysis of RNA products waymore specificwith respect toproduct RNase-catalyzed RNA hydrolysis 2prodsin eqbm 7 RNase: specificity (Most) Enzymes are highly selective catalysts Notice differences between RNase-catalyzed hydrolysis and non-enzymatic alkaline hydrolysis of RNA? Enzymatic (RNasecatalyzed) Substrate Product #2 specificforRnA,notDNA 2'hydroxylactsasanucleophile Non-enzymatic (alkaline hydrolysis) RNAwith...
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This note was uploaded on 04/01/2014 for the course BIOL 201 taught by Professor Chowrira during the Winter '13 term at UBC.

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