201-13-4-Energy Transfer III part B to post (1)

2 1 1 bb 0 a a b c c 2 b a a a b 5 7

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Unformatted text preview: abilized%fibres%to% ET III - 40 examine%mitochondrial%funcCon% IIB A Cyt-C Respiration rate -1 -1 Mo2 (nmol/mg dry wt) (nmol min mg dry wt/min) 12 10 Rotenone 8 Anti-A 6 4 Mal/Pyr TMPD Succinate ADP 2 0 0 10 20 Time (min) 30 40 ET III - 41 2 1 0 * b * a 4 b 0 a a 14 a 4 10 0 c b b b * a * * * * 13 (C) Complex II-IV Temperature ( C) b 1 0.2 1 1 bb ** 0 a a b c c 2 b a * * a a * * b 5 7 1.2a 10 6 1.0 8 5 0.8a 4 0.6 6 3 0.4 2b 4 b 1 0.2 St III / St II b b 4 a b b * * * 18 a a a b b 10 a 12 a a b * b * 10 a * * 8 6 4 2 0 21 (F a b a a c * c b 21 o Temperature ( C) a a a b a a a 6 * b 2 5 c b * * a * c 5 8 6 4 2 13 8 * a ** * * 10 4 a 13 13 21 21 ET III - 42 a b a Temperature o Temperature (oC) ( C) a a 8 14 a a b b 16 a ** * 12 a a 41 0.2 b b 2 0 0.0 0 5 13 21 (D) Complex IV (F) RCR Temperature (oC) 18 10 16 14 0.0 10 * 0 0.0 21 2 13 a b 0.2 a 5 Temperature (oC) (E) State II respiration (malate + pyruvate) (D) Complex IV (F) RCR (C) Complex II-IV (D) Complex IV 1.2 0.4 * * 0.6 (F) RCR (C) (E) StateII-IV Complex II respiration (malate + pyruvate) a b a187 n rate g-1 dry wt) 7 n rate -1 dry wt) 2 a a c 0.4 * A 6 0.8 b 0.6 3 0 0.0 Normoxic Anoxic * o 5 c 166 1.0 145 0.8 a12 a 4 10 0.6 a 83 0.4 62 bb a 5 6 * 4 St III / St II 6 33 0 Normoxic Anoxic 16 0 * Respiration rate (nmol min-1 mg-1 dry wt) b (B) Complex I-IV 18 7 44 * 22 (D) Complex IV 1 * a Respiration rate (nmol min-1 mg-1 dry wt) * St III / St II Respiration rate -1 Respiration -1 dry wt) (nmol min-1 mg-1rate (nmol min mg dry wt) 1 b 55 II Respiration rate b 0 Respiration Mo(nmolmin-1-1mg-1rate wtwt/min) dry (nmol min mg-1 dry wt) 2 (nmol/mg dry ) a b 2 2 a (F) RCR 8 66 Respiration rate Respiration rate (nmol min-1 mg-1 dry wt) (nmol min-1 mg-1 dry wt) Respiration rate (nmol min-1 mg-1 dry wt) a 3 4 * IIB * 77 4 6 2 b b 0.8 10 (B) Complex I-IV (C) Complex II-IV 5 8 3 0.0 1 6 5 0.2 c 2 7 12 0.4 *3 (C) Complex II-IV b St III / St II a Respiration rat (nmol min-1 mg-1 d c Respiration ra (nmol min-1 mg-1 d Respir (nmol min- 3 -1 150 10 100 5 50 0 Citrate synthase Complex I Complex II Complex IV Enzyme activity (nmol min mg ) Normoxic Anoxic Activity (µmol/mg protein/min) -1 -1 -1 EA...
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This note was uploaded on 04/01/2014 for the course BIOL 201 taught by Professor Chowrira during the Winter '13 term at The University of British Columbia.

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