201-13-4-Energy Transfer III part B to post (1)

Adp and pi need to be transported into the matrix

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Unformatted text preview: ansported into the matrix from the cytosol and ATP needs to be transported out to the cytosol after synthesis ! One IMM protein transports ATP in exchange for ADP ! Another IMM protein uses the transport of 1 H+ into the matrix to transport HPi in (HPi = H2PO4-1 = H+ + Pi) ET III - 35 Energy Transfer III! Oxidative Phosphorylation! 3 3 H+ 4 H+ are required to enter the matrix to synthesize 1 ATP (3 through the ATPase + 1 through the HPi transport protein). (Pi) ET III - 36 Mar252013 Energy Transfer III! Experimental techniques to investigate Oxidative Phosphorylation! Oxidative Phosphorylation can be inhibited by inhibiting ETC function e- Complex II B iClickerQuestion#1-- picture:answerB Amytal Rotenone inhibite-mvtinfeeders e- Complex I Q rotenone:lipidsoluble, gointoinnermitochondrialmembrane, blocke-transferbetweencomplexI Complex II A andQ e- -CN CO Antimycin A Complex III c Complex IV turnblue, hemeisdeoxygenated O2 cyanide, carbonmonoxide hemeprostheticgroup CO--bindstohemeprotein locksitindeoxygenatedstate sowhenhemoglobincomes ET III - 37 tolungs,can'tgetridof CO2,can'tgetoxygen Energy Transfer III! Experimental techniques to investigate Oxidative Phosphorylation! Mitochondria are robust organelles for experimental manipulation. Isolated mitochondria versus Inverted Inner Mitochondrial Membrane vesicles I2M2 vesicles ET III - 38 ET III - 39 Energy Transfer III! Experimental techniques to investigate Oxidative Phosphorylation! Mitochondria+from+Anoxia+Acclimated+Turtles+ Dr.+Gina+Galli+ PDF+2009+to+2011+ University+of+Manchester+ Cold%(5°C)%acclimated%turtles.% Two%experimental%groups:% a)  Two%weeks%anoxia%exposure%with%no%access%to%air% b)  Two%weeks%normoxia%with%access%to%air% % Euthanized%animals,%dissected%hearts,%perme...
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This note was uploaded on 04/01/2014 for the course BIOL 201 taught by Professor Chowrira during the Winter '13 term at UBC.

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