201-13-4-Energy Transfer III part B to post (1)

Fifo atp synthase ims matrix et iii 27 energy transfer

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Unformatted text preview: Matrix ET III - 27 Energy Transfer III! Oxidative Phosphorylation! Very Cool Video Compare and contrast: Moran et al. Ch. 12, Sect. 12.9 (pg 375) with http://www.youtube.com/watch?v=PjdPTY1wHdQ Energy Transfer III! Oxidative Phosphorylation! Moran et al. Ch. 12, Sect. 12.9 (pg 375) In the F1 component of the ATP synthase ET III - 28 Energy Transfer III! Oxidative Phosphorylation! ET III 29 Energy Transfer III! Oxidative Phosphorylation! ETC and ATPase operating together produce a steady state Higher [H+] IMS IMM ETC Matrix H+ +++++++ ---------Lower [H+] H+ ATPase Energy Once a gradient is formed and a steady state exists, we can estimate: 1.  Energy produced from movement (facilitated diffusion) of H+ through the ATPase into the matrix. 2.  Threshold energy required to actively transport H+ out of the matrix to the IMS. ET III - 30 Energy Transfer III! Oxidative Phosphorylation! Membrane Starting Compartment #1 Finishing Compartment #2 H+ Threshold energy required (+ΔG) or energy production (-ΔG) associated with the transport of 1 mol of solute across a membrane from Compartment #1 to Compartment #2 ΔGTransport = ΔGConcentration + ΔGElectrical ET III - 31 Energy Transfer III! Oxidative Phosphorylation! For a positively charged ion: If compartment #1 is more +ve than compartment #2 ΔΨ < 0 If compartment #2 is more +ve than compartment #1 ΔΨ > 0 Example: What is the threshold energy required to transport 1 mol of H+ from matrix to IMS if: pHmatrix = 7.8 pHIMS = 7.1 ΔΨ T = 0.17 V ΔGTransport = = 25oC ET III - 32 Energy Transfer III! Oxidative Phosphorylation! ET III - 33 Energy Transfer III! Summary of Oxidative Phosphorylation! eSubstrate: donated H+ lost O from # ATP Expt. Matrix Expected ATP yield consumed P:O NADH Succinate L-3P ET III - 34 Energy Transfer III! Oxidative Phosphorylation! Steady state considerations !  H+ lost from the matrix due to transport and consumption, are then returned to the matrix via the ATP synthase and !  ADP and Pi need to be tr...
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This note was uploaded on 04/01/2014 for the course BIOL 201 taught by Professor Chowrira during the Winter '13 term at UBC.

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