Unformatted text preview: Matrix ET III  27 Energy Transfer III!
Oxidative Phosphorylation! Very Cool Video
Compare and contrast:
Moran et al. Ch. 12, Sect. 12.9 (pg 375)
with
http://www.youtube.com/watch?v=PjdPTY1wHdQ Energy Transfer III!
Oxidative Phosphorylation!
Moran et al. Ch. 12, Sect. 12.9 (pg 375)
In the F1 component of the ATP synthase ET III  28 Energy Transfer III!
Oxidative Phosphorylation! ET III 29 Energy Transfer III!
Oxidative Phosphorylation!
ETC and ATPase operating together produce a steady state
Higher [H+]
IMS
IMM ETC Matrix H+ +++++++
Lower [H+] H+
ATPase Energy Once a gradient is formed and a steady state exists, we can estimate:
1. Energy produced from movement (facilitated diffusion) of H+
through the ATPase into the matrix.
2. Threshold energy required to actively transport H+ out of the
matrix to the IMS.
ET III  30 Energy Transfer III!
Oxidative Phosphorylation!
Membrane Starting
Compartment #1 Finishing
Compartment #2 H+ Threshold energy required (+ΔG) or energy production (ΔG)
associated with the transport of 1 mol of solute across a membrane
from Compartment #1 to Compartment #2 ΔGTransport = ΔGConcentration + ΔGElectrical
ET III  31 Energy Transfer III!
Oxidative Phosphorylation!
For a positively charged ion:
If compartment #1 is more +ve than compartment #2 ΔΨ < 0 If compartment #2 is more +ve than compartment #1 ΔΨ > 0 Example: What is the threshold energy required to transport 1 mol
of H+ from matrix to IMS if:
pHmatrix = 7.8 pHIMS = 7.1 ΔΨ T = 0.17 V ΔGTransport = = 25oC ET III  32 Energy Transfer III!
Oxidative Phosphorylation! ET III  33 Energy Transfer III!
Summary of Oxidative Phosphorylation! eSubstrate: donated H+ lost
O
from
# ATP
Expt.
Matrix Expected ATP yield consumed P:O NADH
Succinate
L3P ET III  34 Energy Transfer III!
Oxidative Phosphorylation!
Steady state considerations
! H+ lost from the matrix due to transport and consumption, are
then returned to the matrix via the ATP synthase
and
! ADP and Pi need to be tr...
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This note was uploaded on 04/01/2014 for the course BIOL 201 taught by Professor Chowrira during the Winter '13 term at UBC.
 Winter '13
 CHOWRIRA

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