201-13-4-Energy Transfer III part B to post (1)

Oxidative phosphorylation oxidative phosphorylation

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Unformatted text preview: ctivityactivity (µmol min mg ) nzyme (µmol/mg protein/min) 15 200 * 0 Complex V ET III - 43 Energy Transfer III! Oxidative Phosphorylation! Oxidative Phosphorylation can be inhibited by inhibiting the H+ electroconcentration gradient formation These inhibitors are called uncouplers (Unc) H+ High [H+] IMS ETC Matrix Low [H+] H+ ET III - 44 Energy Transfer III! Oxidative Phosphorylation! Uncouplers of Oxidative Phosphorylation High [H+] Protonates Unc + H+ Unc-H+ neutralizecomplex, probablynocharge H+ High [H+] IMS Unc ETC Low [H+] H+ eg. 2,4-Dinitrophenol (DNP) Unc transportsH+withoutgenerationofATP, n Matrix Unc-H+ + H+ Lower [H+] Deprotonates ET III - 45 biologyusesthisaswell! Energy Transfer! Thermogensis - Natures use of uncouplers! adiposetissue, fullofmitochondria, usedforgenerationofheat, predominantinnumberoforganisms: smallmammals,(highSA:volume),constantlylosing Brown Fat heat, hibernatingsquirrels, rewarmit infanthumans, shoulderfat, cannotregulate heatverywell White Fat ET III - 46 Energy Transfer! Thermogensis - Natures use of uncouplers! brownfatcells cyclerunning ferventlyfast, inducingfutilecycleofprotons=>point:futilecycling,energytransdu energynotbeingharnessedas inhighpH,dissociates ATP,butbecomesheatgenerat ET organ,allowsheatin III - 47 brownadiposetissue Energy Transfer III! Oxidative Phosphorylation! Oxidative Phosphorylation can be inhibited by inhibiting F1F0-ATP synthase Oligomycin: an antibiotic synthesized by some bacter...
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