# Fish Kill - Exp 14 Procedure Proposal Template Fall 2013...

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Exp. 14 Procedure Proposal Template - Fall 2013 Replace all NON-BOLD text with the information indicated. Please leave the BOLD section headings as they are. Numbered items indicate the desired order of information within a section. This assignment should be typed (size 12 font and double spaced). The only parts that may be hand-written are experimental diagrams and equations/calculations. One assignment should be turned in per pair of partners. Determination of the Cause of a “Fish-Kill” in the Clark Fork of the Columbia River Michelle Nguyen Prachi Karna Lab Section #40 6 November 2013
Introduction When metal ions from groups IA and IIA are dissolved into water, it causes the water to have high salinity and toxicity, which can possibly kill any fish that are living in such a location. This experiment aims to analyze the amount of Na + , K + , Li + , Ca +2 , Ba +2 , Sr +2 , Cu +2 , and Fe +3 ions in a simulated sample of water from the Clark Fork in order to determine whether or not any of these particular ions are killing the fish in the Columbia River. Students will be performing serial dilutions and using emission and absorption spectroscopy to determine the ions found in the lake sample. Equations that will be used for this lab: (M 1 )(V 1 ) = (M 2 )(V 2 ) E = hc / λ E = (6.63 x 10 -34 J s)(3.00 x 10 8 m/s) / (5.89 x 10 -9 m) E = 3.37 x 10 -19 J/atom If the ppm (parts per million) of the Cu2+ and Fe3+ ions are exceed 50-100 ppm and 20-50 ppm respectively, this means the exposure to these ions is making the water toxic and is killing the fish in the river. In order to protect the fish and other living organisms who consume these fish in the Clark Fork in the Columbia River, the water needs to be tested in order make a conclusion as to what is harming the fish in the river. If fish are dying in the river and the cause is not determined, the situation has the potentiality to increase and further harm many more species in the future. This is why it is important to stop the exposure to a metal ion as early as possible if this is found to be the cause. Emission spectroscopy is used for the detection of Group IA and IIA metal ions, which form colorless aqueous solutions. Students will use emission spectroscopy for the unknown colorless solution to detect colors in the solution. The wavelength peaks will determine the specific ions in the unknown solution.