Lecture+14_Beaudin_2014

Rodent myocardial infarction model lineage tracing of

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: be harnessed to repair damage l༆  Proliferating cardiomyocytes l༆  Endogenous cardiac progenitors Is the heart capable of regeneration? l༆  Zebrafish CAN! Is the heart capable of regeneration? l༆  Zebrafish CAN! l༆  Higher levels of proliferating CM at baseline l༆  Genetic “pulse chase “ labeling studies Increased proliferation of pre- existing CM after injury Even though ZF CM regenerate, they do not have an endogenous “cardiac stem cell” Kikuchi et al, Nature 2010 Regeneration in the mammalian heart? Rodent Myocardial Infarction Model Lineage tracing of endogenous progenitors/ cardiomyocytes OR Inject progenitors/stem cells at site of injury and look for incorporation/ cardiomyocyte differentiation/ functional readout Regeneration in the mammalian heart? Neonatal rodent heart l༆  Embryonic heart capable of mounting response to injury l༆  Neonatal mouse heart (prior to septation) more similar to fish heart - Heart beats under lower pressure -   Neonatal cardiomyocytes are still mononucleated and proliferating l༆  Is mechanism same or different from adult cardiac regeneration? Porello et al, Science 2011 Regeneration in the neonatal rodent heart l༆  Two approaches: Genetic pulse- chase BrdU co- labeling – which cell types contribute to regeneration? Porello et al, Science 2011 Regeneration in the neonatal rodent heart Porello et al, Science 2011 BrdU Co- labeling l༆  Cardiomyocytes (Nkx2.5+) are Brdu+ l༆  Cardiomyocytes are proliferating in resected hearts Genetic pulse- labeling l༆  No differences in % lacZ+ myocardium between resected and sham l༆  No replacement of myocardium by unlabeled (L...
View Full Document

This document was uploaded on 03/28/2014.

Ask a homework question - tutors are online