Lecture+14_Beaudin_2014

Cardiomyocytes diversity of cardiac progenitors

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Flexible polymer tubes allows for cell fusion and vascularization Hydrogel scaffolds mimic extracellular matrix and can be pre- seeded with cells inside Cardiac Tissue Engineering: Scaffolding l༆  Examples: hydrogel, nanofibers, alginate, cardiac extracellular matrix l༆  Clinical trials underway in US, Israel Leor et al, JACC 2009 Cardiac Tissue Engineering: Scaffolding • Injection of alginate improves LV function AND reduced remodeling • May support growth and/or survival via paracrine factors, promotion of angiogenesis Tissue Engineering: Requirements for success l༆  Identification/generation of scaffold materials that mimic cardiac niche l༆  Successful “seed” cells l༆  Appropriate biological, mechanical, AND electrical stimulation of seeded cells Tissue Engineering: Successful “seed” cells l༆  Potential sources of cells – l༆  Endogenous l༆  BM- derived cells l༆  Original reports claimed that BM cells could generate l༆  l༆  l༆  l༆  cardiomyocytes!! Since disproven – shown to be cell fusion events Attractive because BM transplants are widely used and safe May exert benecial effects via paracrine effects – promote survival and/or enhance angiogenesis In clinical trials but limited success, particularly with CM recovery l༆  ES/iPS cells Why purify stem cell- derived cardiomyocytes? l༆  Embryonic stem cells (ESCs) and iPS cells potentially represent a virtually unlimited supply of CMs l༆  iPS cells represent putative autologous source l༆  Trials with BM and mSC have had limited success l༆  Little ev...
View Full Document

Ask a homework question - tutors are online