Cardiomyocytes diversity of cardiac progenitors

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Unformatted text preview: Flexible polymer tubes allows for cell fusion and vascularization Hydrogel scaffolds mimic extracellular matrix and can be pre- seeded with cells inside Cardiac Tissue Engineering: Scaffolding l༆  Examples: hydrogel, nanofibers, alginate, cardiac extracellular matrix l༆  Clinical trials underway in US, Israel Leor et al, JACC 2009 Cardiac Tissue Engineering: Scaffolding • Injection of alginate improves LV function AND reduced remodeling • May support growth and/or survival via paracrine factors, promotion of angiogenesis Tissue Engineering: Requirements for success l༆  Identification/generation of scaffold materials that mimic cardiac niche l༆  Successful “seed” cells l༆  Appropriate biological, mechanical, AND electrical stimulation of seeded cells Tissue Engineering: Successful “seed” cells l༆  Potential sources of cells – l༆  Endogenous l༆  BM- derived cells l༆  Original reports claimed that BM cells could generate l༆  l༆  l༆  l༆  cardiomyocytes!! Since disproven – shown to be cell fusion events Attractive because BM transplants are widely used and safe May exert benecial effects via paracrine effects – promote survival and/or enhance angiogenesis In clinical trials but limited success, particularly with CM recovery l༆  ES/iPS cells Why purify stem cell- derived cardiomyocytes? l༆  Embryonic stem cells (ESCs) and iPS cells potentially represent a virtually unlimited supply of CMs l༆  iPS cells represent putative autologous source l༆  Trials with BM and mSC have had limited success l༆  Little ev...
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