Material l smoother surfaces illicit immune response

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Unformatted text preview: er 14C levels than pre- test conditions, confirming DNA synthesis! Regeneration in the human heart l༆  Limited regenerative capacity decreases with age l༆  ~50% of cardiomyocytes have been regenerated during lifespan l༆  No information regarding regeneration from CM or from CPC Limited regeneration and/or proliferation suggests the possibility of augmentation of endogenous processes! Can endogenous repair be augmented? l༆  Pharmacological manipulation of CM cell cycle may be a more attractive alternative to cell or gene therapy: l༆  Stimulation with mitogens l༆  DNA synthesis ≠ mitosis l༆  Hearts don’t get cancer = less sensitive to mitogens! l༆  TISSUE ENGINEERING l༆  Bioengineering scaffolds to assist with endogenous repair! l༆  REGENERATIVE MEDICINE l༆  Exogenous cells for repair! Tissue Engineering Augment the endogenous with the exogenous! l༆  Major objectives of Cardiac Tissue Engineering: 1) Create conditions (scaffolds) that promote homing of CM progenitors to site of injury 2) Improve efficiency of stem cell application to heart injury 3) Build functional 3- D heart tissues Tissue Engineering: Requirements for success l༆  Identification/generation of scaffold materials that mimic cardiac niche l༆  Successful “seed” cells l༆  Appropriate biological, mechanical, AND electrical stimulation of seeded cells Cardiac Tissue Engineering: Scaffolding l༆  Materials onto which cells are seeded and/or home to: l༆  Must be biodegradable Ideally, promote healing as they l༆  Must be biocompatible biodegrade l༆  Surface topology has a greater influence on biocompatibility than material! l༆  Smoother surfaces illicit immune response + fibrotic invasion l༆  Complex surfaces with pores and grooves allow for vascularization and promote regrowth Scaffolding examples Accordion “honeycomb” structure guides cardiomyocyte orientation and native mechanical response...
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This document was uploaded on 03/28/2014.

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