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Unformatted text preview: You devise a method to detect the protein with fluorescence, and
do a FRAP experiment. You bleach a very small area (<1%)of the Golgi, and measure
fluorescence in that area over time. You normalize the values to the initial fluorescence, and get
the graphs below. Giantin is noted by a solid line, and GPP130 by the dashed line.
a) Which protein diffuses faster? Giantin (slope is higher)
b) Draw the expected trace if all of giantin were mobile. Goes up to ~100%
c) Draw the expected trace of giantin if you bleach the whole Golgi apparat...
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