Bioc 212 Lecture 5, 6 2014

Figure 14 25 molecular biology of the cell garland

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Unformatted text preview: e chain down stream is oxidized. Figure 14-25 Molecular Biology of the Cell (© Garland Science 2008) An electrochemical proton gradient Figure 14-13 Molecular Biology of the Cell (© Garland Science 2008) the ATP amde in the mitochondria doesn't pay as much as that made through glycolysis because there is a cost. and the cost is 1 proton, because it has to be transported across the membrane ADP is imported at the same time that we export ATP, so the voltage is the driving force for this process. import of pyruvate requires energy so as we import pyruvate we also co-import a proton, Figure 14-16 Molecular Biology of the Cell (© Garland Science 2008) inorganic phosphate also has a negative charge so this is also co-imported with a proton. the transporter binds both. The electro-chemical gradient across the inner membrane of mitochondria • 2 gradients are generated across the inner membrane: – Voltaic: 180 to 190 mV – pH (osmotic): about 0.5 pH units • Both are put at work – In the oxydative phosphorylation process – In the inport/export of metabolites 31 Figure 14-14 Molecular Biology of the Cell (© Garland Science 2008) Figure 14-15 Molecular Biology of the Cell (© Garland Science 2008) Figure 14-19 Molecular Biology of the Cell (© Garland Science 2008) Chemio-Osmotic coupling an overview • Electrons carry out work to ‘pump’ protons across the inner membrane of the mitochondria • These electrons are donated by NADH, and FADH2 (also others) within the mitochondria • This is done at many steps, while they travel through the ‘wire’: the electron transport (respiratory) chain • The energy stored within this gradient, in turn, is used to generate ATP: Oxidative phosphorylation by the FoF1 ATP synthetase complex 35 Let’s count the NADH & ATPs! (you know you want to...) • What are the net products ... – per glucose, under hypoxic conditions? – per acetyl-coa, in the mitochondria, under normoxic conditions? – per pyruvate? – per...
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This note was uploaded on 04/01/2014 for the course BIOC 212 taught by Professor Dr.young during the Winter '11 term at McGill.

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